Sugar binding to recombinant wild-type and mutant glucokinase monitored by kinetic measurement and tryptophan fluorescence.

Zelent, Bogumil; Odili, Stella; Buettger, Carol; Shiota, Chiyo; Grimsby, Joseph; Taub, Rebecca; Magnuson, Mark A; Vanderkooi, Jane M; Matschinsky, Franz M

Zelent, Bogumil; Odili, Stella; Buettger, Carol; Shiota, Chiyo; Grimsby, Joseph; Taub, Rebecca; Magnuson, Mark A; Vanderkooi, Jane M; Matschinsky, Franz M.

Affiliation

Zelent B; Department of Biochemistry and Biophysics and Diabetes Research Center, University of Pennsylvania, Philadelphia, PA 19104, USA.

Biochem J ; 413(2): 269-80, 2008 Jul 15.

Article in En | MEDLINE | ID: mdl-18370929

Subject(s)

Glucokinase/genetics; Mutation; Recombinant Proteins/chemistry; Tryptophan/chemistry; Binding Sites; Carbohydrates/chemistry; Escherichia coli/metabolism; Glutathione Transferase/metabolism; Humans; Kinetics; Molecular Conformation; Protein Binding; Protein Conformation; Recombinant Fusion Proteins/chemistry; Spectrometry, Fluorescence/methods

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Tryptophan / Recombinant Proteins / Glucokinase / Mutation Limits: Humans Language: En Journal: Biochem J Year: 2008 Document type: Article Affiliation country: United States Country of publication: United kingdom

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