[Detection of the expression level of S100C gene in lung cancer tissue by RT-PCR and construction and identification of prokaryotic expression vector].
Wei Sheng Yan Jiu
; 37(1): 79-81, 2008 Jan.
Article
in Zh
| MEDLINE
| ID: mdl-18421872
ABSTRACT
OBJECTIVE:
To analyze the expression level of S100C gene in lung cancer tissue and to construct prokaryotic expression vector pET30a-S100C in order to select fusion antibody in further study.METHODS:
mRNA expression level of S100C gene in lung cancer tissue and ambient tissue in 26 cases was detected by RT-PCR with p-actin as references. The full-length cDNA of S100C was cloned by RT-PCR. The amplified DNA fragments were ligated into pET30a vector and then transformed into E. coli stain JM109. The positive clones were screened out and indentified.RESULTS:
The expression level of S100C in lung cancer tissue was lower than that in ambient tissue. The difference between the two groups was statistically significant (P < 0.05). The prokaryotic expression victor of S100C was successfully constructed. The sequence homogenuity was up to 100%.CONCLUSION:
The down-regulation expression of S100C gene probably takes important part in the carcinogenisis of lung cancer.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
S100 Proteins
/
Genetic Vectors
/
Lung Neoplasms
Type of study:
Diagnostic_studies
Limits:
Adult
/
Aged
/
Female
/
Humans
/
Male
/
Middle aged
Language:
Zh
Journal:
Wei Sheng Yan Jiu
Journal subject:
SAUDE PUBLICA
Year:
2008
Document type:
Article
Affiliation country:
China
Publication country:
CHINA
/
CN
/
REPUBLIC OF CHINA