[Detection of the expression level of S100C gene in lung cancer tissue by RT-PCR and construction and identification of prokaryotic expression vector].

ABSTRACT

OBJECTIVE: To analyze the expression level of S100C gene in lung cancer tissue and to construct prokaryotic expression vector pET30a-S100C in order to select fusion antibody in further study. METHODS: mRNA expression level of S100C gene in lung cancer tissue and ambient tissue in 26 cases was detected by RT-PCR with p-actin as references. The full-length cDNA of S100C was cloned by RT-PCR. The amplified DNA fragments were ligated into pET30a vector and then transformed into E. coli stain JM109. The positive clones were screened out and indentified. RESULTS: The expression level of S100C in lung cancer tissue was lower than that in ambient tissue. The difference between the two groups was statistically significant (P < 0.05). The prokaryotic expression victor of S100C was successfully constructed. The sequence homogenuity was up to 100%. CONCLUSION: The down-regulation expression of S100C gene probably takes important part in the carcinogenisis of lung cancer.