Evaluation of two commercially available nucleic acid hybridization assays for the detection and typing of human papillomavirus in clinical specimens.
Am J Clin Pathol
; 95(1): 21-9, 1991 Jan.
Article
in En
| MEDLINE
| ID: mdl-1846260
ABSTRACT
Southern blot (Oncor, Gaithersburg, MD) and dot blot (Life Technologies, Gaithersburg, MD) nucleic acid hybridization assays were compared for their ability to detect and type human papillomavirus (HPV) DNA in 50 cervical swab specimens and 11 biopsy specimens. Overall agreement between the two methods was 78.7%. With the use of Southern blot analysis, HPV 6, 11, 16, or 18 was detected in 22 specimens, however, 4 were untypable because of abnormal or smeared band patterns. Dot blot analysis detected HPV 6/11, 16/18, or 31/33/35 in those same 22 specimens and in 9 additional specimens. Eight of the 13 specimens in which HPV was not detected or untypable by Southern blot contained type 31/33/35 by dot blot. Based on convenience of specimen collection and transport, ease of performance and the ability to detect HPV types 31, 33, and 35, the authors are currently using the dot blot assay for the detection and typing of HPV in clinical specimens.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Papillomaviridae
/
DNA, Viral
/
Nucleic Acid Hybridization
Type of study:
Diagnostic_studies
/
Evaluation_studies
Limits:
Adolescent
/
Adult
/
Aged
/
Female
/
Humans
/
Middle aged
Language:
En
Journal:
Am J Clin Pathol
Year:
1991
Document type:
Article