Assessing new bone formation in neonatal calvarial organ cultures.
Methods Mol Biol
; 455: 37-50, 2008.
Article
in En
| MEDLINE
| ID: mdl-18463809
ABSTRACT
Bone formation is a complex process, and testing anabolic effects on the skeleton of agents is slow and expensive in animals. Neonatal mouse calvariae cultured ex vivo show strong anabolic or catabolic bone responses to 1-week treatments and can be analyzed by quantitative histomorphometry. Changes in new bone area and osteoblast number caused by added proteins, drugs, or transfected genes can be quantified and analyzed for statistical significance. The organ cultures preserve much of the skeletal architecture and cellular diversity present in vivo and offer greater physiological relevance than cell lines studied in vitro.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Organ Culture Techniques
/
Osteogenesis
/
Skull
/
Biological Assay
Limits:
Animals
/
Pregnancy
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2008
Document type:
Article
Affiliation country:
United States