An okadaic acid-sensitive phosphatase negatively controls the cyclin degradation pathway in amphibian eggs.
Mol Cell Biol
; 11(2): 1171-5, 1991 Feb.
Article
in En
| MEDLINE
| ID: mdl-1846666
ABSTRACT
Inhibition of okadaic acid-sensitive phosphatases released the cyclin degradation pathway from its inhibited state in extracts prepared from unfertilized Xenopus eggs arrested at the second meiotic metaphase. It also switched on cyclin protease activity in a permanent fashion in interphase extracts prepared from activated eggs. Even after cdc2 kinase inactivation, microinjection of okadaic acid-treated interphase extracts pushed G2-arrested recipient oocytes into the M phase, suggesting that the phosphatase inhibitor stabilizes the activity of an unidentified factor which shares in common with cdc2 kinase the maturation-promoting factor activity.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oocytes
/
Cyclins
/
Phosphoprotein Phosphatases
/
Ethers, Cyclic
Type of study:
Diagnostic_studies
/
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Mol Cell Biol
Year:
1991
Document type:
Article
Affiliation country:
France