Improving the yield of soluble 6xHis-tagged interferon-alpha via the addition of repressor of the araBAD promoter system in Escherichia coli.
Biotechnol Lett
; 30(9): 1577-82, 2008 Sep.
Article
in En
| MEDLINE
| ID: mdl-18488147
ABSTRACT
The inhibition of inclusion body formation in Escherichia coli by the addition of alpha-D-glucopyranoside or D-fucose after induction improved the purification yield of soluble recombinant interferon-alpha. When D-fucose was added after induction, more soluble 6xHis-tagged interferon-alpha could be purified compared to when methyl alpha-D-glucopyranoside was added. It was shown that, on the basis of 1 mg dry cell weight, 16.6 microg of soluble 6xHis-tagged interferon-alpha was purified when D-fucose was added after induction and 6 ml nickel-chelated agarose gel column was used. This was about 15 times greater than when induction only was performed and 1 ml nickel-chelated agarose gel was used.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oligopeptides
/
Operon
/
Repressor Proteins
/
Recombinant Proteins
/
Promoter Regions, Genetic
/
Interferon-alpha
/
Escherichia coli
/
Histidine
Limits:
Humans
Language:
En
Journal:
Biotechnol Lett
Year:
2008
Document type:
Article