Differential expression of LAR tyrosine phosphatase in the rat developing molar tooth germ.
Arch Oral Biol
; 53(10): 947-53, 2008 Oct.
Article
in En
| MEDLINE
| ID: mdl-18555975
ABSTRACT
OBJECTIVE:
This study examined the molecules implicated in the cytodifferentiation of dental hard tissue cells.METHODS:
Rat pups at postnatal days 4, 7 and 10 were used. Differential display-polymerase chain reaction (DD-PCR), Western blot and immunofluorescent localisation were performed to search differentially expressed genes in tooth development.RESULTS:
Leukocyte-common antigen-related tyrosine phosphatase (lar-tp) was differentially detected between the rat maxillary 2nd and 3rd molar germs on postnatal day 10, which were at the dental hard tissue formation and cap/early bell developmental stages, respectively. Both the mRNA and protein expression levels of lar-tp were higher in the 3rd molar germs than in the 2nd. In addition, the levels in the 2nd molar germs at postnatal days 4, 7 and 10, which corresponded to the early/late bell, crown and root stages, respectively, decreased in a time dependent manner. The immunoreactivity against intracellular P-subunit of lar-tp was detected in the ameloblasts and odontoblasts as well as in the undifferentiated inner enamel epithelia and dental papilla cells. However, strong immunoreactivity against extracellular E-subunit was observed only in the undifferentiated inner enamel epithelia and dental papilla cells in the 3rd molar germs and in the stratum intermedium in the 2nd molar germs.CONCLUSION:
This is the first identification of lar-tp in the molar tooth development and suggests that this molecule may be involved in the cytodifferentiation of dental hard tissue cells.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Tooth Germ
/
Receptor-Like Protein Tyrosine Phosphatases, Class 2
/
Molar
Limits:
Animals
Language:
En
Journal:
Arch Oral Biol
Year:
2008
Document type:
Article
Affiliation country:
South Korea