Variation in homeodomain DNA binding revealed by high-resolution analysis of sequence preferences.
Cell
; 133(7): 1266-76, 2008 Jun 27.
Article
in En
| MEDLINE
| ID: mdl-18585359
ABSTRACT
Most homeodomains are unique within a genome, yet many are highly conserved across vast evolutionary distances, implying strong selection on their precise DNA-binding specificities. We determined the binding preferences of the majority (168) of mouse homeodomains to all possible 8-base sequences, revealing rich and complex patterns of sequence specificity and showing that there are at least 65 distinct homeodomain DNA-binding activities. We developed a computational system that successfully predicts binding sites for homeodomain proteins as distant from mouse as Drosophila and C. elegans, and we infer full 8-mer binding profiles for the majority of known animal homeodomains. Our results provide an unprecedented level of resolution in the analysis of this simple domain structure and suggest that variation in sequence recognition may be a factor in its functional diversity and evolutionary success.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
DNA
/
Homeodomain Proteins
Type of study:
Prognostic_studies
Aspects:
Patient_preference
Limits:
Animals
Language:
En
Journal:
Cell
Year:
2008
Document type:
Article
Affiliation country:
United States