Efficient system for biotinylated recombinant Ets-1 production in Escherichia coli: a useful tool for studying interactions between Ets-1 and its partners.

Laitem, Clélia; Choul-Li, Souhaila; Baillat, David; Bègue, Agnès; Aumercier, Marc

Laitem, Clélia; Choul-Li, Souhaila; Baillat, David; Bègue, Agnès; Aumercier, Marc.

Affiliation

Laitem C; CNRS Unité Mixte de Recherche 8161, Institut de Biologie de Lille, Institut Pasteur de Lille, Université de Lille 1, Lille Cedex, France.

Protein Expr Purif ; 62(1): 53-63, 2008 Nov.

Article in En | MEDLINE | ID: mdl-18639639

Subject(s)

Escherichia coli/genetics; Proto-Oncogene Protein c-ets-1/biosynthesis; Recombinant Proteins/biosynthesis; Binding Sites; Biotinylation; Cells, Cultured; DNA/metabolism; Electrophoretic Mobility Shift Assay; Escherichia coli/metabolism; Humans; Jurkat Cells; Kinetics; Models, Biological; Proto-Oncogene Protein c-ets-1/genetics; Proto-Oncogene Protein c-ets-1/isolation & purification; Recombinant Proteins/genetics; Recombinant Proteins/isolation & purification; Surface Plasmon Resonance; Transfection

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Recombinant Proteins / Escherichia coli / Proto-Oncogene Protein c-ets-1 Type of study: Evaluation_studies / Prognostic_studies / Qualitative_research Limits: Humans Language: En Journal: Protein Expr Purif Journal subject: BIOLOGIA MOLECULAR Year: 2008 Document type: Article Affiliation country: France

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