Assessment of the diagnostic potential of Immmunocapture-PCR and Immuno-PCR for Citrus Variegated Chlorosis.
J Microbiol Methods
; 75(2): 302-7, 2008 Oct.
Article
in En
| MEDLINE
| ID: mdl-18656503
ABSTRACT
Xylella fastidiosa causes significant losses in many economically important crops. An efficient pathogen detection system is critical for epidemiology studies, particularly when large sample size is involved. In this study we report the development of immunomolecular assays like Immmunocapture-PCR and Immuno-PCR for direct detection of X. fastidiosa without DNA isolation. Whereas the reactivity of ELISA and PCR ranged from 10(6) to 10(4) bacterial cells, the IC-PCR sensitivity was up to 10(3) and the detection limit of I-PCR was up to 10(1) bacterial cells. These methods can use either plant sample extracts or cultivated media, and show no cross reaction for any other endophytic citrus-bacteria. Therefore, IC-PCR and I-PCR assays provide an alternative for quick and very sensitive methods to screening X. fastidiosa, with the advantage of not requiring any concentration or DNA purification steps while still allowing an accurate diagnosis of CVC.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plant Diseases
/
Enzyme-Linked Immunosorbent Assay
/
Polymerase Chain Reaction
/
Citrus sinensis
/
Xylella
/
Antibodies, Bacterial
Type of study:
Diagnostic_studies
/
Evaluation_studies
Language:
En
Journal:
J Microbiol Methods
Year:
2008
Document type:
Article
Affiliation country:
Brazil