Photopolymerization as an innovative detection technique for low-density microarrays.
Biotechniques
; 45(2): 179-82, 184-6, 2008 Aug.
Article
in En
| MEDLINE
| ID: mdl-18687067
ABSTRACT
One limitation that accounts in part for the scarcity of commercially available diagnostic microarrays is the expense associated with fluorescence detection. Here we present a colorimetric method based on photopolymerization as an "on-chip" signal amplification technique. Proof of principle experiments are detailed and followed by the use of a simple influenza microarray to demonstrate the technique for the first time with clinical samples. The advantages of this new technique include rapid (<5 min) signal amplification ( approximately 105) in ambient conditions for both DNA and protein microarrays, low reagent cost (<$1 per assay), visual or inexpensive detection, and signal preservation for at least two years under ambient conditions.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oligonucleotide Array Sequence Analysis
/
Protein Array Analysis
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
En
Journal:
Biotechniques
Year:
2008
Document type:
Article
Affiliation country:
United States