Analysis of CRE-mediated recombination driven by myosin light chain 1/3 regulatory elements in embryonic and adult skeletal muscle: a tool to study fiber specification.
Genesis
; 46(8): 424-30, 2008 Aug.
Article
in En
| MEDLINE
| ID: mdl-18693277
ABSTRACT
An increasing number of genes have been implicated in skeletal muscle fiber diversity. To study the contribution of diverse genetic elements to the regulation of fiber-type composition, we generated a transgenic mouse in which CRE recombinase expression is driven by muscle-specific regulatory sequences of the myosin light chain 1/3 locus (MLC). Using ROSA26 conditional reporter mice, we detected expression of the MLC-Cre transgene starting from embryonic day 12.5 (E12.5). By E15, recombination was detected in all muscle-derived structures. Immunohistochemical analysis revealed CRE activity was restricted to fast-twitch (type II) and excluded from slow-twitch (type I) fibers of skeletal muscle. The MLC-Cre transgenic mouse can be used in conjunction with conditional alleles to study both developmental patterning and maintenance of fast fiber-type phenotypes.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Regulatory Sequences, Nucleic Acid
/
Muscle, Skeletal
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Muscle Fibers, Fast-Twitch
/
Myosin Light Chains
Limits:
Animals
Language:
En
Journal:
Genesis
Journal subject:
BIOLOGIA MOLECULAR
Year:
2008
Document type:
Article