Lack of Na(+),K (+)-ATPase expression in intercalated cells may be compensated by Na(+)-ATPase: a study on MDCK - C11 cells.
Cell Mol Life Sci
; 65(19): 3093-9, 2008 Oct.
Article
in En
| MEDLINE
| ID: mdl-18759127
ABSTRACT
The lack of Na(+),K(+)-ATPase expression in intercalated cells (IC) is an intriguing condition due to its fundamental role in cellular homeostasis. In order to better understand this question we compared the activities of Na(+),K(+)-ATPase and Na(+)-ATPase in two MDCK cell clones the C11, with IC characteristics, and the C7, with principal cells (PC) characteristics. The Na(+),K(+)-ATPase activity found in C11 cells is far lower than in C7 cells and the expression of its beta-subunit is similar in both cells. On the other hand, a subset of C11 without alpha-subunit expression has been found. In C11 cells the Na(+)-ATPase activity is higher than that of the Na(+),K(+)-ATPase, and it is increased by medium alkalinization, suggesting that it could account for the cellular Na(+)-homeostasis. Although further studies are necessary for a better understanding of these findings, the presence of Na(+)-ATPase may explain the adequate survival of cells that lack Na(+),K(+)-ATPase.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Adenosine Triphosphatases
/
Sodium-Potassium-Exchanging ATPase
/
Cation Transport Proteins
/
Nephrons
Limits:
Animals
Language:
En
Journal:
Cell Mol Life Sci
Journal subject:
BIOLOGIA MOLECULAR
Year:
2008
Document type:
Article
Affiliation country:
Brazil