Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of hypothetical protein SCO4226 from Streptomyces coelicolor A3(2).
Acta Crystallogr Sect F Struct Biol Cryst Commun
; 64(Pt 9): 847-50, 2008 Sep 01.
Article
in En
| MEDLINE
| ID: mdl-18765920
ABSTRACT
A non-Pfam hypothetical protein SCO4226 of molecular weight 9 kDa from Streptomyces coelicolor A3(2) was overexpressed in Escherichia coli and the purified recombinant protein was crystallized using the sitting-drop vapour-diffusion method. An X-ray diffraction data set was collected to 2.0 A resolution. The crystal belonged to space group P2(1), with unit-cell parameters a = 29.67, b = 67.00, c = 34.43 A, alpha = gamma = 90.00, beta = 94.26 degrees .
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Bacterial Proteins
/
Cloning, Molecular
/
Streptomyces coelicolor
Language:
En
Journal:
Acta Crystallogr Sect F Struct Biol Cryst Commun
Year:
2008
Document type:
Article