High-level expression and characterization of an anti-VEGF165 single-chain variable fragment (scFv) by small ubiquitin-related modifier fusion in Escherichia coli.

Ye, Tingmei; Lin, Zhihua; Lei, Huanzong

Ye, Tingmei; Lin, Zhihua; Lei, Huanzong.

Affiliation

Ye T; Department of Biology, School of Chemistry and Life Sciences, Lishui University, Lishui, 323000, Zhejiang, People's Republic of China.

Appl Microbiol Biotechnol ; 81(2): 311-7, 2008 Nov.

Article in En | MEDLINE | ID: mdl-18795288

Subject(s)

Antibodies/metabolism; Immunoglobulin Variable Region/metabolism; Recombinant Fusion Proteins/metabolism; SUMO-1 Protein/metabolism; Vascular Endothelial Growth Factor A/immunology; Antibodies/genetics; Cell Proliferation/drug effects; Cells, Cultured; Chromatography, Affinity; Endothelial Cells/drug effects; Escherichia coli/genetics; Gene Expression; Humans; Immunoglobulin Variable Region/genetics; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/isolation & purification; SUMO-1 Protein/genetics; Sequence Analysis, Protein; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Recombinant Fusion Proteins / Immunoglobulin Variable Region / SUMO-1 Protein / Vascular Endothelial Growth Factor A / Antibodies Limits: Humans Language: En Journal: Appl Microbiol Biotechnol Year: 2008 Document type: Article

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