Altered immunodominance hierarchies of influenza A virus-specific H-2(b)-restricted CD8+ T cells in the absence of terminal deoxynucleotidyl transferase.
Immunol Invest
; 37(7): 714-25, 2008.
Article
in En
| MEDLINE
| ID: mdl-18821218
ABSTRACT
Immunodominance is considered an obstacle to successful T cell-based vaccination, and constant efforts are made to uncover the underlying mechanisms for this phenomenon. We have examined the contribution of terminal deoxynucleotidyl transferase (TdT), whose function accounts for approximately 90% of T cell receptor diversity, to dominance hierarchies of H-2(b)-restricted flu-specific T(CD8+). Using intracellular cytokine staining to quantitatively detect epitope-specific T(CD8+), we demonstrate that TdT-deficient mice exhibit a distinct hierarchical pattern in their primary and recall T(CD8+) responses to influenza A viruses, which results from skewed responsiveness towards select influenza epitopes. Our data establish a link between TdT and immunodominance in H-2(b)-restricted antiviral T(CD8+) responses.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Influenza A virus
/
H-2 Antigens
/
Orthomyxoviridae Infections
/
CD8-Positive T-Lymphocytes
/
Epitopes, T-Lymphocyte
/
DNA Nucleotidylexotransferase
Limits:
Animals
Language:
En
Journal:
Immunol Invest
Journal subject:
ALERGIA E IMUNOLOGIA
Year:
2008
Document type:
Article
Affiliation country:
Canada