An isocratic fluorescence HPLC assay for the monitoring of l-asparaginase activity and l-asparagine depletion in children receiving E. colil-asparaginase for the treatment of acute lymphoblastic leukaemia.

Nath, Christa E; Dallapozza, Luciano; Eslick, Adam E; Misra, Ashish; Carr, Deborah; Earl, John W

Nath, Christa E; Dallapozza, Luciano; Eslick, Adam E; Misra, Ashish; Carr, Deborah; Earl, John W.

Affiliation

Nath CE; Department of Biochemistry, The Children's Hospital at Westmead, Westmead, NSW, Australia. christan@chw.edu.au

Biomed Chromatogr ; 23(2): 152-9, 2009 Feb.

Article in En | MEDLINE | ID: mdl-18823071

Subject(s)

Antineoplastic Agents/metabolism; Asparaginase/metabolism; Asparagine/metabolism; Chromatography, High Pressure Liquid/methods; Precursor Cell Lymphoblastic Leukemia-Lymphoma/drug therapy; Adolescent; Antineoplastic Agents/blood; Antineoplastic Agents/therapeutic use; Asparaginase/blood; Asparaginase/therapeutic use; Asparagine/blood; Aspartic Acid/blood; Aspartic Acid/metabolism; Child; Child, Preschool; Drug Stability; Escherichia coli Proteins/therapeutic use; Fluorescence; Glutamic Acid/blood; Glutamic Acid/metabolism; Glutamine/analysis; Glutamine/blood; Glutamine/metabolism; Homoserine/analysis; Humans; Least-Squares Analysis; Reference Standards; Reproducibility of Results; Sensitivity and Specificity

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Asparaginase / Asparagine / Chromatography, High Pressure Liquid / Precursor Cell Lymphoblastic Leukemia-Lymphoma / Antineoplastic Agents Type of study: Diagnostic_studies Limits: Adolescent / Child / Child, preschool / Humans Language: En Journal: Biomed Chromatogr Year: 2009 Document type: Article Affiliation country: Australia

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