A novel, generic and effective method for the rapid purification of G protein-coupled receptors.

Magnin, Thierry; Fiez-Vandal, Cédric; Potier, Noëlle; Coquard, Aline; Leray, Isabelle; Steffan, Tania; Logez, Christel; Alkhalfioui, Fatima; Pattus, Franc; Wagner, Renaud

Magnin, Thierry; Fiez-Vandal, Cédric; Potier, Noëlle; Coquard, Aline; Leray, Isabelle; Steffan, Tania; Logez, Christel; Alkhalfioui, Fatima; Pattus, Franc; Wagner, Renaud.

Affiliation

Magnin T; LC1-UMR 7175, Institut Gilbert Laustriat, Pôle API, Bd Sébastien Brant, BP 10413, 67412 Illkirch Cedex, France. magnin@esbs.u-strasbg.fr

Protein Expr Purif ; 64(1): 1-7, 2009 Mar.

Article in En | MEDLINE | ID: mdl-18835448

Subject(s)

Receptors, G-Protein-Coupled/isolation & purification; Biotinylation; Endopeptidases/metabolism; Humans; Microspheres; Models, Biological; Pichia/genetics; Protein Structure, Tertiary; Receptors, G-Protein-Coupled/genetics; Receptors, G-Protein-Coupled/metabolism; Recombinant Fusion Proteins/chemistry; Recombinant Fusion Proteins/isolation & purification; Recombinant Fusion Proteins/metabolism; Sepharose/metabolism; Solubility; Streptavidin/isolation & purification; Streptavidin/metabolism

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Receptors, G-Protein-Coupled Limits: Humans Language: En Journal: Protein Expr Purif Journal subject: BIOLOGIA MOLECULAR Year: 2009 Document type: Article Affiliation country: France

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