Spectroscopic properties and bacteriochlorophyll c isomer composition of extramembranous light-harvesting complexes in the green sulfur photosynthetic bacterium Chlorobium tepidum and its CT0388-deleted mutant under vitamin B12-limited conditions.
Photochem Photobiol Sci
; 7(10): 1210-5, 2008 Oct.
Article
in En
| MEDLINE
| ID: mdl-18846285
ABSTRACT
The effects of exogenous vitamin B12 on the green sulfur photosynthetic bacterium Chlorobium (Chl.) tepidum were examined. Wild-type cells and mutant cells lacking a gene CT0388 (denoted as VB0388) of Chl.tepidum were grown in liquid cultures containing different concentrations of vitamin B12. The VB0388 cells hardly grew in vitamin B12-limited media, indicating that the product of CT0388 actually played an important role in vitamin B12 biosynthesis in Chl. tepidum. Both wild-type and VB0388 cells in vitamin B12-limited media exhibited absorption bands and CD signals at the Qy region that were shifted to a shorter wavelength than those of cells grown in normal media. BChl c isomers that had S-stereochemistry at the 3(1)-position tended to increase in Chl. tepidum grown in vitamin B12-limited media.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Bacterial Proteins
/
Bacteriochlorophylls
/
Vitamin B 12
/
Chlorobium
/
Light-Harvesting Protein Complexes
/
Mutation
Language:
En
Journal:
Photochem Photobiol Sci
Journal subject:
BIOLOGIA
/
QUIMICA
Year:
2008
Document type:
Article
Affiliation country:
Japan