The quantitation of rabies-specific antibodies. II. Factors affecting the sensitivity of the haemagglutination inhibition test.
Biologicals
; 19(2): 97-101, 1991 Apr.
Article
in En
| MEDLINE
| ID: mdl-1888501
ABSTRACT
Various factors affecting the HAI test for the quantitation of rabies-specific antibodies have been evaluated with a view to obtaining maximum sensitivity and reproducibility in tests using tissue culture antigens prepared in vero cells and concentrated by dialysis. Goose erythrocytes treated with proteolytic enzyme bromelian at a concentration of 0.025% were much more susceptible to HA than those that were untreated or erythrocytes treated with neuraminidase. In addition, other parameters like the use of a phosphate buffered saline (PBS) as a diluent at pH 6.2, incubation at 0-4 degrees C for 1.5-3 h were found to be most critical for achieving maximum HA activity. To remove non-specific inhibitors, serum samples were treated with aerosil, acetone in combination or alone. Of the 73 serum samples tested, removal of non-specific inhibitors by aerosil alone occurred in up to 54.79% of the samples, whereas using acetone-aerosil treatment followed by adsorption with goose erythrocytes, the inhibitors were removed in 98.67% of the samples to a level that was undetectable at the 14 starting dilution in the HAI test.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Rabies virus
/
Hemagglutination Inhibition Tests
/
Antibodies, Viral
Type of study:
Diagnostic_studies
/
Evaluation_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Biologicals
Journal subject:
ALERGIA E IMUNOLOGIA
Year:
1991
Document type:
Article
Affiliation country:
India