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Comparison of alcohol dehydrogenase expression in Drosophila melanogaster and D. simulans.
Thomson, M S; Jacobson, J W; Laurie, C C.
Affiliation
  • Thomson MS; Department of Zoology, Duke University, Durham, North Carolina 27706.
Mol Biol Evol ; 8(1): 31-48, 1991 Jan.
Article in En | MEDLINE | ID: mdl-1900559
Alcohol dehydrogenase (ADH) gene expression was analyzed in Drosophila melanogaster and its sibling species D. simulans. The levels of ADH activity, ADH-cross-reacting material (CRM), and ADH-mRNA were analyzed for several strains of each species, which derive from diverse geographic locations around the world. There is considerable quantitative variation in ADH activity, CRM level, and RNA level among strains within species at all developmental stages. However, the only consistent differences between the two species are in pupal RNA level and in late-adult activity and CRM level. Late-adult melanogaster flies that are homozygous for the Slow allozyme have approximately twice the level of ADH activity and CRM as do simulans flies. The regression of activity on CRM over strains is highly significant and essentially the same for each species, which means that most, if not all, of the activity difference between the species is due to a difference in concentration of the ADH protein. In contrast, there is no significant regression of CRM level on mRNA level in adults of either species; nor is there a significant difference in RNA level between species. Therefore, the difference in ADH protein concentration is not due to RNA template availability. Thus, the interspecific difference in ADH level in adults must be due either to a difference in the rate of translation of the two RNAs or to a difference in protein stability.
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Collection: 01-internacional Database: MEDLINE Main subject: Alcohol Dehydrogenase / Gene Expression / Drosophila / Drosophila melanogaster Type of study: Diagnostic_studies Limits: Animals Language: En Journal: Mol Biol Evol Journal subject: BIOLOGIA MOLECULAR Year: 1991 Document type: Article Country of publication: United States
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Collection: 01-internacional Database: MEDLINE Main subject: Alcohol Dehydrogenase / Gene Expression / Drosophila / Drosophila melanogaster Type of study: Diagnostic_studies Limits: Animals Language: En Journal: Mol Biol Evol Journal subject: BIOLOGIA MOLECULAR Year: 1991 Document type: Article Country of publication: United States