Determination of a hydrophilic paclitaxel derivative, 7-xylosyl-10-deacetylpaclitaxel in rat plasma by LC-MS/MS.
Biomed Chromatogr
; 23(5): 472-9, 2009 May.
Article
in En
| MEDLINE
| ID: mdl-19016230
ABSTRACT
A LC-MS/MS method for the determination of a hydrophilic paclitaxel derivative 7-xylosyl-10-deacetylpaclitaxel in rat plasma was developed to evaluate the pharmacokinetics of 7-xylosyl-10-deacetylpaclitaxel in the rats. 7-Xylosyl-10-deacetylpaclitaxel and docetaxel (IS for 7-xylosyl-10-deacetylpaclitaxel) were extracted from rat plasma with acetic ether and analyzed on a Hypersil C(18 )column (4.6 x 150 mm i.d., particle size 5 microm) with the mobile phase of ACN/0.05% formic acid (5050, v/v). The analytes were detected using an ESI MS/MS in the multiple reaction monitoring mode. The standard curves for 7-xylosyl-10-deacetylpaclitaxel in plasma were linear (r > 0.999) over the concentration range of 2.0-1000 ng/mL with a weighting of 1/concentration(2). The method showed a satisfactory sensitivity (2.0 ng/mL using 50 microL plasma), precision (CV
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Chromatography, High Pressure Liquid
/
Paclitaxel
/
Tandem Mass Spectrometry
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
En
Journal:
Biomed Chromatogr
Year:
2009
Document type:
Article