Heat shock response of the archaebacterium Methanococcus voltae.
J Bacteriol
; 173(10): 3224-7, 1991 May.
Article
in En
| MEDLINE
| ID: mdl-1902465
ABSTRACT
The general properties of the heat shock response of the archaebacterium Methanococcus voltae were characterized. The induction of 11 heat shock proteins, with apparent molecular weights ranging from 18,000 to 90,000, occurred optimally at 40 to 50 degrees C. Some of the heat shock proteins were preferentially enriched in either the soluble (cytoplasm) or particulate (membrane) fraction. Alternative stresses (ethanol, hydrogen peroxide, NaCl) stimulated the synthesis of subsets of the heat shock proteins as well as unique proteins. Western blot (immunoblot) analysis, in which antisera to Escherichia coli heat shock proteins (DnaK and GroEL) were used, did not detect any immunologically cross-reactive proteins. In addition, Southern blot analysis did not reveal any homology between M. voltae and four highly conserved heat shock genes, mopB and dnaK from E. coli and hsp70 genes from Drosophila species and Saccharomyces cerevisiae.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Archaea
/
Heat-Shock Proteins
Language:
En
Journal:
J Bacteriol
Year:
1991
Document type:
Article
Affiliation country:
Canada