Detection of Marteilia refringens using nested PCR and in situ hybridisation in Chamelea gallina from the Balearic Islands (Spain).
Dis Aquat Organ
; 82(1): 79-87, 2008 Oct 16.
Article
in En
| MEDLINE
| ID: mdl-19062756
ABSTRACT
In the course of a histopathological survey performed to discover the cause of mass mortality of the striped clam Chamelea gallina in the Balearic Islands (Spain, Mediterranean Sea), we detected a Marteilia-like parasite in 3 clams. Molecular methods were applied to identify the parasite. DNA extracted from a paraffin block was used to carry out a PCR assay for Marteilia refringens detection based on a rDNA sequence of the parasite (the intergenic spacer of ribosomal genes, IGS). The nucleotide sequence of the IGS amplified fragment and the positive signal obtained by in situ hybridisation analysis with a M. refringens-specific probe allowed us to confirm the presence of this parasite in the digestive gland tissue of C. gallina.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Polymerase Chain Reaction
/
Bivalvia
/
In Situ Hybridization
/
Eukaryota
Type of study:
Diagnostic_studies
Limits:
Animals
Country/Region as subject:
Europa
Language:
En
Journal:
Dis Aquat Organ
Journal subject:
BIOLOGIA
/
MEDICINA VETERINARIA
/
MICROBIOLOGIA
Year:
2008
Document type:
Article
Affiliation country:
Spain