Cloning, chromosomal localization, SNP detection and association analysis of the porcine IRS-1 gene.
Mol Biol Rep
; 36(8): 2087-92, 2009 Nov.
Article
in En
| MEDLINE
| ID: mdl-19093226
ABSTRACT
Insulin receptor substrate-1(IRS-1) gene is one member of the Insulin receptor substrate (IRS) gene family, which plays an important role in mediating the growth of skeletal muscle and the molecular metabolism of type 2 diabetes. Here, we cloned a 3,573 bp fragment of the partial CDS sequence of porcine IRS-1 gene by in silicon cloning strategy and RT-PCR method. The porcine IRS-1 gene was assigned to SSC15q25 by using IMpRH. Sequencing of PCR products from Duroc and Tibetan pig breeds identified one SNP in exon 1 of porcine IRS-1 gene (C3257A polymorphisms). Association analysis of genotypes with the growth traits, anatomy traits, meat quality traits and physiological biochemical indexes traits showed that different genotypes at locus 3,257 of IRS-1 have significant differences in carcass straight length in pigs (P = 0.0102 \ 0.05).
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Swine
/
Insulin Receptor Substrate Proteins
/
Genetic Association Studies
Type of study:
Diagnostic_studies
/
Risk_factors_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Mol Biol Rep
Year:
2009
Document type:
Article
Affiliation country:
China