Reconstruction of three-dimensional human skin model composed of dendritic cells, keratinocytes and fibroblasts utilizing a handy scaffold of collagen vitrigel membrane.

Uchino, T; Takezawa, T; Ikarashi, Y

Uchino, T; Takezawa, T; Ikarashi, Y.

Affiliation

Uchino T; National Institute of Health Sciences, Division of Environmental Chemistry, 1-18-1 Kamiyoga, Setaga-ku, Tokyo 158-8501, Japan. uchino@nihs.go.jp

Toxicol In Vitro ; 23(2): 333-7, 2009 Mar.

Article in En | MEDLINE | ID: mdl-19121381

Subject(s)

Dendritic Cells/metabolism; Fibroblasts/metabolism; Keratinocytes/metabolism; Membranes, Artificial; Skin, Artificial; Tissue Scaffolds; Animal Testing Alternatives; B7-2 Antigen/metabolism; Cell Culture Techniques/methods; Cells, Cultured; Coculture Techniques/methods; Collagen Type I/chemistry; Cytokines/metabolism; Dendritic Cells/cytology; Dendritic Cells/drug effects; Fibroblasts/cytology; Fibroblasts/drug effects; Gels; Humans; Irritants/toxicity; Keratinocytes/cytology; Keratinocytes/drug effects; Models, Biological; Time Factors

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Dendritic Cells / Keratinocytes / Skin, Artificial / Tissue Scaffolds / Fibroblasts / Membranes, Artificial Type of study: Prognostic_studies Limits: Humans Language: En Journal: Toxicol In Vitro Journal subject: TOXICOLOGIA Year: 2009 Document type: Article Affiliation country: Japan Country of publication: United kingdom

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