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Novel method for determination of substance P levels in unextracted human plasma by using acidification.
Campbell, Donald E; Bruckner, Paula; Tustin, Nancy B; Tustin, Richard; Douglas, Steven D.
Affiliation
  • Campbell DE; Joseph Stokes Jr. Research Institute at the Children's Hospital of Philadelphia, Department of Pediatrics, University of Pennsylvania School of Medicine, USA. campbelld@email.chop.edu
Clin Vaccine Immunol ; 16(4): 594-6, 2009 Apr.
Article in En | MEDLINE | ID: mdl-19193832
Substance P (SP) is a member of the tachykinin family and has an important role in immune responses. SP is detectable in plasma in a free and bound state. Simple modification of a commercially available SP enzyme-linked immunosorbent assay allows the dissociation and capture of plasma SP without solid-phase extraction.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Plasma / Substance P Limits: Humans Language: En Journal: Clin Vaccine Immunol Journal subject: ALERGIA E IMUNOLOGIA / TECNICAS E PROCEDIMENTOS DE LABORATORIO Year: 2009 Document type: Article Affiliation country: United States Country of publication: United States

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Plasma / Substance P Limits: Humans Language: En Journal: Clin Vaccine Immunol Journal subject: ALERGIA E IMUNOLOGIA / TECNICAS E PROCEDIMENTOS DE LABORATORIO Year: 2009 Document type: Article Affiliation country: United States Country of publication: United States