Leukotriene D4 stimulates the migration but not proliferation of endothelial cells mediated by the cysteinyl leukotriene cyslt(1) receptor via the extracellular signal-regulated kinase pathway.
J Pharmacol Sci
; 109(2): 285-92, 2009 Feb.
Article
in En
| MEDLINE
| ID: mdl-19234368
ABSTRACT
The actions of cysteinyl leukotrienes (CysLTs) are mediated by activating CysLT receptors, CysLT(1), and CysLT(2). The CysLT(1) receptor mediates vascular responses to CysLTs; however, its effect on the proliferation and migration of endothelial cells is not clarified. To determine this effect, we observed proliferation and migration in EA.hy926 cells, a human endothelial cell line, and the involvement of activation of mitogen-activated protein kinases (MAPKs). We found that LTD(4) did not affect the proliferation, but significantly stimulated the migration of endothelial cells. LTD(4) also induced the phosphorylation of extracellular signal-regulated kinase (ERK) 1/2, but not those of p38 or JNK. The LTD(4)-induced migration and ERK1/2 phosphorylation were blocked by the CysLT(1)-receptor antagonist montelukast and the dual antagonist Bay u9773, but not by the CysLT(2)-receptor antagonist Bay cysLT2; the migration was also inhibited by the ERK1/2 inhibitor U0126. Our findings indicate that LTD(4) stimulates the CysLT(1) receptor-mediated migration of endothelial cells; this may be regulated by the ERK1/2 pathway.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cell Movement
/
Receptors, Leukotriene
/
Leukotriene D4
/
Extracellular Signal-Regulated MAP Kinases
/
Cell Proliferation
Limits:
Humans
Language:
En
Journal:
J Pharmacol Sci
Journal subject:
FARMACOLOGIA
Year:
2009
Document type:
Article
Affiliation country:
China