Molecular cloning and characterization of porcine homeodomain transcription factor Msx1.
J Reprod Dev
; 55(3): 278-82, 2009 Jun.
Article
in En
| MEDLINE
| ID: mdl-19262020
ABSTRACT
We cloned a porcine ortholog of homeodomain transcription factor Msx1 from the porcine pituitary cDNA library. The amino acid sequence of Msx1 shows high conservation among mammalian species. RT-PCR for porcine fetal and postnatal pituitaries showed that Msx1 is already expressed at early fetal day 40, decreases to a low level before birth and then remarkably decreases after birth. On the other hand, Msx1 expression was observed in all pituitary-derived cell line tested, with most in a gonadotrope lineage LbetaT4. Transfection assay demonstrated that Msx1 markedly repressed the basal Cga and Fshb gene expression, while Lhb expression was affected slightly. Taken together, Msx1 may play a role in repressing gene expression in the fetal and postnatal periods.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Swine
/
Cloning, Molecular
/
MSX1 Transcription Factor
Limits:
Animals
Language:
En
Journal:
J Reprod Dev
Journal subject:
MEDICINA REPRODUTIVA
Year:
2009
Document type:
Article