Rapid molecular genotyping and clonal complex assignment of Staphylococcus aureus isolates by PCR coupled to electrospray ionization-mass spectrometry.
J Clin Microbiol
; 47(6): 1733-41, 2009 Jun.
Article
in En
| MEDLINE
| ID: mdl-19297593
ABSTRACT
We describe a high-throughput assay using PCR coupled to electrospray ionization-mass spectrometry (PCR/ESI-MS) to determine the genotypes of Staphylococcus aureus isolates. The primer sets used in the PCR/ESI-MS assay were designed to amplify the same genes analyzed in multilocus sequence typing (MLST). The method was used to identify the clonal complex and USA type of each isolate and is suitable for use in a clinical or public-health setting. The method was validated using a panel of diverse isolates from the Centers for Disease Control and Prevention that were previously characterized by MLST and pulsed-field gel electrophoresis (PFGE). Clinical isolates from two geographically distinct hospitals were characterized, and the clustering results were in agreement with those for repetitive-element PCR and PFGE. The PCR/ESI-MS method enables genotyping of over 180 samples of S. aureus per day in an automated fashion.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Staphylococcal Infections
/
Staphylococcus aureus
/
Polymerase Chain Reaction
/
Bacterial Typing Techniques
/
Spectrometry, Mass, Electrospray Ionization
Type of study:
Diagnostic_studies
/
Evaluation_studies
Limits:
Humans
Country/Region as subject:
America do norte
Language:
En
Journal:
J Clin Microbiol
Year:
2009
Document type:
Article
Affiliation country:
United States