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Developmental toxicity of Ochratoxin A in rat embryo midbrain micromass cultures.
Wilk-Zasadna, Iwona; Minta, Maria.
Affiliation
  • Wilk-Zasadna I; Department of Pharmacology & Toxicology, National Veterinary Research Institute, Al. Partyzantow 57, 24-100 Pulawy, Poland.
  • Minta M; Department of Pharmacology & Toxicology, National Veterinary Research Institute, Al. Partyzantow 57, 24-100 Pulawy, Poland.
Int J Mol Sci ; 10(1): 37-49, 2009 Jan.
Article in En | MEDLINE | ID: mdl-19333434
Embryonic midbrain micromass cultures were exposed for five days to ochratoxin A (OTA) at seven concentrations (ranging from 0.16 to 10 microg/mL). Cell viability was assessed in neutral red uptake test (NRU), and differentiation - by immunoenzymatic determination of structural proteins (beta(III)-tubulin, MAP2, GFAP) expression level as well as by computer image analysis. Dose dependent decrease in cell number and differentiation was observed. Concentration-response curves were analysed and the mean inhibition concentrations (microg/mL) for cytotoxicity (IC(50)) and differentiation (ID(50)) were calculated. There were no significant differences in the sensitivity of neurons in early and late stage of differentiation and astrocytes to the toxic activity of this compound. For all endpoints ID(50) value was very low (< 10 microg/mL) so OTA was classified as a strong teratogen. IC(50)/ ID(50) ratios <2 pointed out that with harmful action of OTA the basic cytotoxicity should be connected.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Mesencephalon / Carcinogens / Astrocytes / Neurons / Ochratoxins Limits: Animals Language: En Journal: Int J Mol Sci Year: 2009 Document type: Article Affiliation country: Poland Country of publication: Switzerland

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Mesencephalon / Carcinogens / Astrocytes / Neurons / Ochratoxins Limits: Animals Language: En Journal: Int J Mol Sci Year: 2009 Document type: Article Affiliation country: Poland Country of publication: Switzerland