Ion exchange chromatography of monoclonal antibodies: effect of resin ligand density on dynamic binding capacity.
J Chromatogr A
; 1216(20): 4366-71, 2009 May 15.
Article
in En
| MEDLINE
| ID: mdl-19344903
Dynamic binding capacity (DBC) of a monoclonal antibody on agarose based strong cation exchange resins is determined as a function of resin ligand density, apparent pore size of the base matrix, and protein charge. The maximum DBC is found to be unaffected by resin ligand density, apparent pore size, or protein charge within the tested range. The critical conductivity (conductivity at maximum DBC) is seen to vary with ligand density. It is hypothesized that the maximum DBC is determined by the effective size of the proteins and the proximity to which they can approach one another. Once a certain minimum resin ligand density is supplied, additional ligand is not beneficial in terms of resin capacity. Additional ligand can provide flexibility in designing ion exchange resins for a particular application as the critical conductivity could be matched to the feedstock conductivity and it may also affect the selectivity.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Chromatography, Ion Exchange
/
Ion Exchange Resins
/
Antibodies, Monoclonal
Type of study:
Evaluation_studies
Language:
En
Journal:
J Chromatogr A
Year:
2009
Document type:
Article
Affiliation country:
United States
Country of publication:
Netherlands