Up-regulation of protease-activated receptor-1 in diabetic glomerulosclerosis.
Biochem Biophys Res Commun
; 384(2): 173-9, 2009 Jun 26.
Article
in En
| MEDLINE
| ID: mdl-19401193
ABSTRACT
Patients with diabetes are under a hypercoagulable state leading to generation of thrombin. It is not known whether thrombin plays a role in the progression of diabetic nephropathy. We analyzed gene expression of two thrombin receptors, protease-activated receptor-1 (PAR-1) and PAR-4 in the kidney of diabetic db/db mice. Mice developed hyperglycemia from 7 to 10 weeks of age and showed renal abnormalities such as mesangial expansion and urinary albumin excretion at 10 weeks of age. PAR-1 mRNA was up-regulated in isolated glomeruli in db/db mice compared with age-matched db/m littermates, but PAR-4 mRNA was not. In situ hybridization studies showed that PAR-1 mRNA was detected mainly at the glomerulus, and that intensive signals were observed in mesangial cells and podocytes. The up-regulation of PAR-1 in glomeruli in diabetic mice may play a role in the progression of glomerulosclerosis and abnormal urinary albumin excretion in diabetic nephropathy.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Receptor, PAR-1
/
Diabetic Nephropathies
/
Kidney Glomerulus
Limits:
Animals
Language:
En
Journal:
Biochem Biophys Res Commun
Year:
2009
Document type:
Article
Affiliation country:
Japan