Assessment of the total effective xenoestrogen burden in extracts of human placentas.
Biomarkers
; 14(5): 271-7, 2009 Aug.
Article
in En
| MEDLINE
| ID: mdl-19476411
ABSTRACT
We have standardized a method to assess the total effective xenoestrogen burden (TEXB) in human placentas by the extraction and separation by high-performance liquid chromatography of two fractions containing lipophilic xenoestrogens (alpha) and endogenous hormones (beta), followed by assessing their estrogenicity in MCF-7 breast cancer cell-based E-Screen and Yeast Estrogen Screen (YES) bioassays. The means of TEXB alpha concentrations (in estradiol equivalent (Eeq) units) were 1.32 and 0.77 Eeq pM g(-1) placenta in the E-Screen and YES, respectively; TEXB beta concentrations were 6.97 and 11.56 Eeq pM g(-1) placenta, respectively. The interclass correlation coefficient was low and a fair level of agreement was observed after kappa test correction. According to the E-Screen and YES, TEXB alpha was > or = LOD in 70.0 and 55.0% of the placentas and 92.5 and 82.5% in beta, respectively. Although both bioassays can be recommended for assessing TEXB, there is greater experience with the use of the E-Screen for estrogenic assessment after extensive extraction of complex human matrices.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Placenta
/
Biological Assay
/
Cell Extracts
/
Estrogens
Limits:
Adult
/
Female
/
Humans
/
Pregnancy
Language:
En
Journal:
Biomarkers
Journal subject:
BIOQUIMICA
Year:
2009
Document type:
Article
Affiliation country:
Spain