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Expanded multilocus sequence typing for burkholderia species.
Spilker, Theodore; Baldwin, Adam; Bumford, Amy; Dowson, Chris G; Mahenthiralingam, Eshwar; LiPuma, John J.
Affiliation
  • Spilker T; Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, 48109-0646, USA.
J Clin Microbiol ; 47(8): 2607-10, 2009 Aug.
Article in En | MEDLINE | ID: mdl-19494070
PCR primers targeting loci in the current Burkholderia cepacia complex multilocus sequence typing scheme were redesigned to (i) more reliably amplify these loci from B. cepacia complex species, (ii) amplify these same loci from additional Burkholderia species, and (iii) enable the use of a single primer set per locus for both amplification and DNA sequencing.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA, Bacterial / DNA Fingerprinting / Bacterial Typing Techniques / Burkholderia Type of study: Evaluation_studies Language: En Journal: J Clin Microbiol Year: 2009 Document type: Article Affiliation country: United States Country of publication: United States

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA, Bacterial / DNA Fingerprinting / Bacterial Typing Techniques / Burkholderia Type of study: Evaluation_studies Language: En Journal: J Clin Microbiol Year: 2009 Document type: Article Affiliation country: United States Country of publication: United States