Efficient production of human beta-defensin 2 (HBD2) in Escherichia coli.
Protein Pept Lett
; 16(6): 668-76, 2009.
Article
in En
| MEDLINE
| ID: mdl-19519528
ABSTRACT
Human beta-defensin 2 (HBD2) has been shown to interact with pathogenic bacteria and components of the mammalian innate and adaptive immune response. We describe a quick and reliable method for the production of HBD2 in Escherichia coli. HBD2 was expressed as an insoluble fusion, chemically cleaved and oxidised to give a single, folded HBD2 beta-isoform. The purified peptide was analysed by high resolution mass spectrometry, displayed a well-dispersed (1)H NMR spectrum, was a chemoattractant to HEK293 cells expressing CCR6 and acted as an antimicrobial agent against E. coli, P. aeruginosa, C. albicans and S. aureus.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Beta-Defensins
/
Escherichia coli
/
Anti-Infective Agents
Limits:
Humans
Language:
En
Journal:
Protein Pept Lett
Journal subject:
BIOQUIMICA
Year:
2009
Document type:
Article
Affiliation country:
United kingdom