[Simultaneous determination of four alkaloids in Lindera aggregate by high performance liquid chromatography].
Zhongguo Zhong Yao Za Zhi
; 34(5): 583-6, 2009 Mar.
Article
in Zh
| MEDLINE
| ID: mdl-19526789
ABSTRACT
OBJECTIVE:
To develop an HPLC method for simultaneous determination of four major alkaloids in Lindera aggregate.METHOD:
The analysis was carried out on an Agilent ZORBAX SB-C18 column (4.6 mm x 250 mm, 5 microm) with gradient elution using acetonitrile-water (containing 0. 15% diethylamine, adjusted to pH = 3.0 with acetic acid) as mobile phase. Flow rate was 1.0 mL x min(-1) and the detection wavelength was at 289 nm.RESULT:
The calibration curves were linear over the range of 0.428-8.560 microg for boldine, 2.122-31.83 microg for norboldine, 0.760-15.20 microg for reticuline and 0.020 4-0.400 8 microg for linderegatine, respectively. The average recoveries were 99.18% for boldine, 101.0% for norboldine, 100.3% for reticuline and 99.17% for linderegatine, respectively. with RSD not more than 3.0%.CONCLUSION:
The described method is reliable and convenient and could be used for the quality control of Lindera aggregate.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Chromatography, High Pressure Liquid
/
Lindera
/
Alkaloids
Type of study:
Evaluation_studies
Language:
Zh
Journal:
Zhongguo Zhong Yao Za Zhi
Journal subject:
FARMACOLOGIA
/
TERAPIAS COMPLEMENTARES
Year:
2009
Document type:
Article
Affiliation country:
China