[Analysis different transcriptional factors in different phenotype endometrial cancer cells].
Zhonghua Fu Chan Ke Za Zhi
; 44(3): 209-13, 2009 Mar.
Article
in Zh
| MEDLINE
| ID: mdl-19570448
ABSTRACT
OBJECTIVE:
To analysis the activity of transcriptional factors in endometrial cancer cell lines with different estrogen receptor subtypes.METHODS:
The mRNA levels of estrogen receptor (ER) was detected by quantitative RT-PCR, and the activity of transcriptional factors was also analysed by 345-channel protein/DNA array in RL-952 (the expression status of ERalpha and ERbeta both positive), HEC-1A [ERalpha (+/-), while ERbeta negative] and HEC-1B (ERalpha and ERbeta both negative). The transcription factors of NFkappaBp65 and p38MAPK with different activity were tested by enzyme-linked immunosorbent assay (ELISA) to confirm the results of protein/DNA array.RESULTS:
The mRNA levels of ERalpha in RL-952, HEC-1A and HEC-1B were (6780+/-282), (684+/-84) and (168+/-38) copy/ng, respectively. Among 345 candidate transcriptional factors, there were 28 factors associated with ER status. Compared with RL-952 cells, 13 transcriptional activity factors were concomitantly up-regulation, while 15 concomitantly down-regulation in HEC-1A and HEC-1B cells. Transcriptional activities of TTF (1)-1, NRF-1, TCE were significantly correlated with the high-expression status of ERalpha mRNA (r=0.523, P=0.037), while RFX123 and Ikaros were significantly correlated with the low-expression status of ERalpha mRNA (r=-0.312, P=0.041).CONCLUSION:
Transcriptional factors of TTF (1)-1, NRF-1, TCE may be associated with ER-mediated signal pathway, while RFX123 and Ikaros may be associated with non ER-mediated signal pathway in endometrial cancer.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Endometrial Neoplasms
/
P38 Mitogen-Activated Protein Kinases
/
Estrogen Receptor alpha
/
Estrogen Receptor beta
/
Transcription Factor RelA
Limits:
Female
/
Humans
Language:
Zh
Journal:
Zhonghua Fu Chan Ke Za Zhi
Year:
2009
Document type:
Article
Affiliation country:
China