UNA (unlocked nucleic acid): a flexible RNA mimic that allows engineering of nucleic acid duplex stability.
Bioorg Med Chem
; 17(15): 5420-5, 2009 Aug 01.
Article
in En
| MEDLINE
| ID: mdl-19604699
ABSTRACT
UNA (unlocked nucleic acid) monomers are acyclic derivatives of RNA lacking the C2'-C3'-bond of the ribose ring of RNA. Synthesis of phosphoramidite UNA building blocks of the nucleobases adenine, cytosine, guanine, and uracil is described herein together with their incorporation into RNA strands. UNA monomers additively decrease nucleic acid duplex stability and can be positioned strategically to induce either lack of discrimination of mismatches, that is, universal base behavior, or increased discrimination of mismatches, that is, improved hybridization specificity. UNA-modified RNA duplexes are shown to structurally mimic unmodified RNA duplexes by CD spectroscopy.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Organophosphorus Compounds
/
Uracil
/
RNA
/
Adenine
/
Cytosine
/
Guanine
Language:
En
Journal:
Bioorg Med Chem
Journal subject:
BIOQUIMICA
/
QUIMICA
Year:
2009
Document type:
Article
Affiliation country:
Denmark