Effects of FGF-2 and EGF removal on the differentiation of mouse neural precursor cells.
An Acad Bras Cienc
; 81(3): 443-52, 2009 Sep.
Article
in En
| MEDLINE
| ID: mdl-19722014
ABSTRACT
Cell therapy for neurological disorders has advanced, and neural precursor cells (NPC) may become the ideal candidates for neural transplantation in a wide range of diseases. However, additional work has to be done to determine either the ideal culture environment for NPC expansion in vitro, without altering their plasticity, or the FGF-2 and EGF mechanisms of cell signaling in neurospheres growth, survival and differentiation. In this work we evaluated mouse neurospheres cultured with and without FGF-2 and EGF containing medium and showed that those growth factors are responsible for NPC proliferation. It is also demonstrated that endogenous production of growth factors shifts from FGF-2 to IGF-1/PDGFb upon EGF and FGF-2 withdrawal. Mouse NPC cultured in suspension showed different patterns of neuronal localization (core versus shell) for both EGF and FGF-2 withdrawal and control groups. Taken together, these results show that EGF and FGF-2 removal play an important role in NPC differentiation and may contribute to a better understanding of mechanisms of NPC differentiation. Our findings suggest that depriving NPC of growth factors prior to grafting might enhance their chance to effectively integrate into the host.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Stem Cells
/
Cell Differentiation
/
Fibroblast Growth Factor 2
/
Epidermal Growth Factor
/
Neuronal Plasticity
/
Neurons
Limits:
Animals
Language:
En
Journal:
An Acad Bras Cienc
Year:
2009
Document type:
Article
Affiliation country:
Brazil