Mitigation of acute ultraviolet B radiation-mediated damages by baicalin in mouse skin.
Photodermatol Photoimmunol Photomed
; 25(5): 250-8, 2009 Oct.
Article
in En
| MEDLINE
| ID: mdl-19747244
ABSTRACT
BACKGROUND:
Solar ultraviolet (UV) irradiation, in particular UVB with a wavelength range between 290 and 320 nm, induces different hazardous effects on the skin, including sunburn, photoaging and cancer. Protection against sun-induced damage is therefore a highly desirable goal. Chemoprevention is being investigated as a potential approach for the management of UV damages including skin cancer.AIM:
In this study, to determine the relevance of our in vitro findings to in vivo situations, we assessed the effects of baicalin on UVB-mediated damages in mice skin.METHODS:
Balb/C hairless mice were topically pretreated (24 h before UVB) or post-treated (5 min after UVB) with baicalin (1 mg/cm(2) skin area/mouse/100 microl acetone) and were exposed to UVB 24 h later (180 mJ/cm(2)). The animals were sacrificed 1 and 24 h after the UVB exposure. Skin edema, histopathology changes, hydrogen peroxide (H2O2) and cyclobutane pyrimidine dimers (CPDs)-positive cells were assessed to determine the UVB-induced photodamage.RESULTS:
Our data demonstrated that a topical application of baicalin, either as a pretreatment or as a post-treatment, resulted in a significant decrease in UVB mediated increases in skin edema, skin hyperplasia and infiltration of leukocytes. Further, baicalin treatments (pre and post) also resulted in a significant decrease in UVB mediated (1) generation of H2O2 and (2) formation of DNA photolesions CPDs.CONCLUSION:
Based on these data, we suggest that baicalin could be developed as an agent for the management of conditions elicited by UV exposure including skin cancer.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Skin
/
Ultraviolet Rays
/
Flavonoids
Limits:
Animals
Language:
En
Journal:
Photodermatol Photoimmunol Photomed
Journal subject:
ALERGIA E IMUNOLOGIA
/
DERMATOLOGIA
Year:
2009
Document type:
Article
Affiliation country:
China