Bioactive modification of silicon surface using self-assembled hydrophobins from Pleurotus ostreatus.
Eur Phys J E Soft Matter
; 30(2): 181-5, 2009 Oct.
Article
in En
| MEDLINE
| ID: mdl-19763645
ABSTRACT
A crystalline silicon surface can be made biocompatible and chemically stable by a self-assembled biofilm of proteins, the hydrophobins (HFBs) purified from the fungus Pleurotus ostreatus. The protein-modified silicon surface shows an improvement in wettability and is suitable for immobilization of other proteins. Two different proteins were successfully immobilized on the HFBs-coated chips the bovine serum albumin and an enzyme, a laccase, which retains its catalytic activity even when bound on the chip. Variable-angle spectroscopic ellipsometry (VASE), water contact angle (WCA), and fluorescence measurements demonstrated that the proposed approach in silicon surface bioactivation is a feasible strategy for the fabrication of a new class of hybrid devices.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Silicon
/
Fungal Proteins
/
Pleurotus
Language:
En
Journal:
Eur Phys J E Soft Matter
Journal subject:
BIOFISICA
Year:
2009
Document type:
Article
Affiliation country:
Italy
Publication country:
FR
/
FRANCE
/
FRANCIA
/
FRANÇA