PTH-induced internalization of apical membrane NaPi2a: role of actin and myosin VI.
Am J Physiol Cell Physiol
; 297(6): C1339-46, 2009 Dec.
Article
in En
| MEDLINE
| ID: mdl-19776390
ABSTRACT
Parathyroid hormone (PTH) plays a critical role in the regulation of renal phosphorous homeostasis by altering the levels of the sodium-phosphate cotransporter NaPi2a in the brush border membrane (BBM) of renal proximal tubular cells. While details of the molecular events of PTH-induced internalization of NaPi2a are emerging, the precise events governing NaPi2a removal from brush border microvilli in response to PTH remain to be fully determined. Here we use a novel application of total internal reflection fluorescence microscopy to examine how PTH induces movement of NaPi2a out of brush border microvilli in living cells in real time. We show that a dynamic actin cytoskeleton is required for NaPi2a removal from the BBM in response to PTH. In addition, we demonstrate that a myosin motor that has previously been shown to be coregulated with NaPi2a, myosin VI, is necessary for PTH-induced removal of NaPi2a from BBM microvilli.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Parathyroid Hormone
/
Cell Membrane
/
Actins
/
Myosin Heavy Chains
/
Sodium-Phosphate Cotransporter Proteins, Type IIa
/
Kidney Tubules, Proximal
Limits:
Animals
Language:
En
Journal:
Am J Physiol Cell Physiol
Journal subject:
FISIOLOGIA
Year:
2009
Document type:
Article
Affiliation country:
United States