The genome of cyprinid herpesvirus 3 encodes 40 proteins incorporated in mature virions.
J Gen Virol
; 91(Pt 2): 452-62, 2010 Feb.
Article
in En
| MEDLINE
| ID: mdl-19846671
ABSTRACT
Koi herpesvirus, also known as cyprinid herpesvirus 3 (CyHV-3), is the aetiological agent of an emerging and mortal disease in common and koi carp. CyHV-3 virions present the characteristic morphology of other members of the order Herpesvirales, being composed of an envelope, a capsid containing the genome and a tegument. This study identified CyHV-3 structural proteins and the corresponding encoding genes using liquid chromatography tandem mass spectrometry-based proteomic approaches. In addition, exponentially modified protein abundance index analyses were used to estimate the relative abundance of the identified proteins in CyHV-3 virions. These analyses resulted in the identification of 40 structural proteins, which were classified based on bioinformatic analyses as capsid (three), envelope (13), tegument (two) and unclassified (22) structural proteins. Finally, a search for host proteins in purified CyHV-3 virions indicated the potential incorporation of up to 18 distinct cellular proteins. The identification of the proteins incorporated into CyHV-3 virions and determination of the viral genes encoding these proteins are key milestones for further fundamental and applied research on this virus.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Virion
/
Viruses
/
Viral Structural Proteins
/
Genome, Viral
/
DNA Virus Infections
/
Fish Diseases
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
J Gen Virol
Year:
2010
Document type:
Article
Affiliation country:
Belgium