Cloning, expression in Pichia pastoris, and characterization of a thermostable GH5 mannan endo-1,4-beta-mannosidase from Aspergillus niger BK01.

Do, Bien-Cuong; Dang, Thi-Thu; Berrin, Jean-Guy; Haltrich, Dietmar; To, Kim-Anh; Sigoillot, Jean-Claude; Yamabhai, Montarop

Do, Bien-Cuong; Dang, Thi-Thu; Berrin, Jean-Guy; Haltrich, Dietmar; To, Kim-Anh; Sigoillot, Jean-Claude; Yamabhai, Montarop.

Affiliation

Do BC; School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Avenue, Nakhon Ratchasima 30000, Thailand.

Microb Cell Fact ; 8: 59, 2009 Nov 13.

Article in En | MEDLINE | ID: mdl-19912637

Subject(s)

Aspergillus niger/enzymology; Mannosidases/metabolism; Pichia/metabolism; Amino Acid Sequence; Cloning, Molecular; Electrophoresis, Polyacrylamide Gel; Glycosylation; Half-Life; Hydrogen-Ion Concentration; Kinetics; Mannans/metabolism; Mannosidases/genetics; Mannosidases/isolation & purification; Molecular Sequence Data; Pichia/genetics; Recombinant Proteins/genetics; Recombinant Proteins/isolation & purification; Recombinant Proteins/metabolism; Substrate Specificity; Temperature

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Pichia / Aspergillus niger / Mannosidases Language: En Journal: Microb Cell Fact Journal subject: BIOTECNOLOGIA / MICROBIOLOGIA Year: 2009 Document type: Article Affiliation country: Thailand Country of publication: United kingdom

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