Enzyme-mediated assimilation of DNA-functionalized single-walled carbon nanotubes.
Langmuir
; 26(2): 613-7, 2010 Jan 19.
Article
in En
| MEDLINE
| ID: mdl-19957946
ABSTRACT
When pyrimidine-functionalized carbon nanotubes were incubated with single-stranded DNA ligase, formations of macroscopic aggregates were observed. Wet-cell transmission electron microscopy imaging revealed that the nanotubes were radially bound to form a 3D latticelike structure. These structures were not observed in control reactions lacking ligase or adenosine triphosphate. Raman spectroscopy analysis revealed no spectra indicative of carbon nanotubes in ligase-unamended controls; however, spectra were observed in radial breathing mode and in the G and G' bands in reactions containing ligase. Furthermore, the addition of deoxyribonuclease to the ligated reactions dispersed the aggregates, and a reduction in Raman spectral intensity was observed.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
DNA Ligases
/
DNA
/
Nanotubes, Carbon
Language:
En
Journal:
Langmuir
Journal subject:
QUIMICA
Year:
2010
Document type:
Article
Affiliation country:
United States