Evaluation of curcumin and cisplatin-induced DNA damage in PC12 cells by the alkaline comet assay.
Hum Exp Toxicol
; 29(8): 635-43, 2010 Aug.
Article
in En
| MEDLINE
| ID: mdl-20051457
ABSTRACT
A very appropriate method for antigenotoxicity evaluation of antioxidants is the comet assay, since this analytical method detects initial DNA lesions that are still subject to repair; in other words, lesions that are very associated to damages resulting from the generation and subsequent action of reactive species. However, a solid evaluation should be developed in order to avoid inexact interpretations. In our study, besides the association of curcumin with cisplatin, curcumin and cisplatin agents were also tested separately. Classical genotoxic compounds, when tested by the comet assay, present an increase in the nucleoid tail; however, the cisplatin treatment has resulted in a decrease of DNA migration. This was an expected effect, as the cross-links between cisplatin and DNA decrease the DNA electrophoretic mobility. A similar effect was observed with the curcumin treatment, which decreased the nucleoid tail. Such effect was not expected and reinforced the necessity of including in the study, separate treatment groups with potentially antigenotoxic substances. The comet assay results have been analyzed using specific software for image analysis, as well as the classical visual analysis, and we have observed that the effect of decrease in DNA electrophoretic mobility was more easily observed when the data were analyzed by the software.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
DNA Damage
/
Cisplatin
/
Comet Assay
/
Curcumin
/
Antineoplastic Agents
Limits:
Animals
Language:
En
Journal:
Hum Exp Toxicol
Journal subject:
TOXICOLOGIA
Year:
2010
Document type:
Article
Affiliation country:
Brazil