Purification and functional characterization of a Camelid-like single-domain antimycotic antibody by engineering in affinity tag.

Kabir, M Enamul; Krishnaswamy, Senthilkumar; Miyamoto, Masahiko; Furuichi, Yasuhiro; Komiyama, Tadazumi

Kabir, M Enamul; Krishnaswamy, Senthilkumar; Miyamoto, Masahiko; Furuichi, Yasuhiro; Komiyama, Tadazumi.

Affiliation

Kabir ME; Department of Biochemistry, Faculty of Pharmaceutical Sciences, Niigata University of Pharmacy and Applied Life Sciences, 265-1 Higashijima, Niigata 956-8603, Japan.

Protein Expr Purif ; 72(1): 59-65, 2010 Jul.

Article in En | MEDLINE | ID: mdl-20060473

Subject(s)

Antibodies, Fungal/isolation & purification; Antifungal Agents/isolation & purification; Antifungal Agents/pharmacology; Cloning, Molecular; Immunoglobulin Variable Region/isolation & purification; Immunoglobulin Variable Region/pharmacology; Animals; Antibodies, Fungal/chemistry; Antibodies, Fungal/immunology; Antibodies, Fungal/pharmacology; Antifungal Agents/immunology; Antifungal Agents/metabolism; Blotting, Western; Candida/drug effects; Chromatography, Affinity; Electrophoresis, Polyacrylamide Gel; Escherichia coli/genetics; Gene Expression; Immunoglobulin Variable Region/genetics; Immunoglobulin Variable Region/immunology; Mutagenesis; Protein Structure, Tertiary; Recombinant Proteins/genetics; Recombinant Proteins/immunology; Recombinant Proteins/isolation & purification; Recombinant Proteins/pharmacology; Ruminants; Saccharomyces/drug effects

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Immunoglobulin Variable Region / Cloning, Molecular / Antibodies, Fungal / Antifungal Agents Limits: Animals Language: En Journal: Protein Expr Purif Journal subject: BIOLOGIA MOLECULAR Year: 2010 Document type: Article Affiliation country: Japan Country of publication: United States

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