Surrobodies with functional tails.
J Mol Biol
; 397(1): 352-60, 2010 Mar 19.
Article
in En
| MEDLINE
| ID: mdl-20100493
ABSTRACT
Surrobodies(2) are a unique type of binding protein based on the pre-B-cell receptor (pre-BCR). The pre-BCR is transiently expressed during development of the antibody repertoire. Unlike heterotetrameric canonical antibodies that are composed of identical pairs of heavy and light chains, the pre-BCR is a heterohexameric complex composed of identical pairs of heavy chains that are each paired with a two-subunit surrogate light chain (SLC). The SLC contains nonimmunoglobulin-like peptide extensions on each of the two SLC components. This arrangement provides unique opportunities for protein engineering by functional derivatization of these nonimmunoglobulin-like tails. Here we report recombinant fusions to these tails with either a fully active cytokine or with single-chain variable fragment (scFv) domains to generate Surrobodies with unique functions or Surrobodies that are bispecific with respect to targeted binding.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Recombinant Fusion Proteins
/
Protein Engineering
/
Immunoglobulin Light Chains, Surrogate
Limits:
Animals
/
Humans
Language:
En
Journal:
J Mol Biol
Year:
2010
Document type:
Article
Affiliation country:
United States