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Microsomal prostaglandin E synthase-1 and cyclooxygenase-2 are both required for ischaemic excitotoxicity.
Ikeda-Matsuo, Y; Hirayama, Y; Ota, A; Uematsu, S; Akira, S; Sasaki, Y.
Affiliation
  • Ikeda-Matsuo Y; Kitasato University, Tokyo, Japan. matsuoy@pharm.kitasato-u.ac.jp
Br J Pharmacol ; 159(5): 1174-86, 2010 Mar.
Article in En | MEDLINE | ID: mdl-20128796
BACKGROUND AND PURPOSE: Although both microsomal prostaglandin E synthase (mPGES)-1 and cyclooxygenase (COX)-2 are critical factors in stroke injury, but the interactions between these enzymes in the ischaemic brain is still obscure. This study examines the hypothesis that mPGES-1 activity is required for COX-2 to cause neuronal damage in ischaemic injury. EXPERIMENTAL APPROACH: We used a glutamate-induced excitotoxicity model in cultures of rat or mouse hippocampal slices and a mouse middle cerebral artery occlusion-reperfusion model in vivo. The effect of a COX-2 inhibitor on neuronal damage in mPGES-1 knockout (KO) mice was compared with that in wild-type (WT) mice. KEY RESULTS: In rat hippocampal slices, glutamate-induced excitotoxicity, as well as prostaglandin (PG) E(2) production and PGES activation, was significantly attenuated by either MK-886 or NS-398, inhibitors of mPGES-1 and COX-2 respectively; however, co-application of these inhibitors had neither an additive nor a synergistic effect. The protective effect of NS-398 on the excitotoxicity observed in WT slices was completely abolished in mPGES-1 KO slices, which showed less excitotoxicity than WT slices. In the transient focal ischaemia model, mPGES-1 and COX-2 were co-localized in the infarct region of the cortex. Injection of NS-398 reduced not only ischaemic PGE(2) production, but also ischaemic injuries in WT mice, but not in mPGES-1 KO mice, which showed less dysfunction than WT mice. CONCLUSION AND IMPLICATIONS: Microsomal prostaglandin E synthase-1 and COX-2 are co-induced by excess glutamate in ischaemic brain. These enzymes are co-localized and act together to exacerbate stroke injury, by excessive PGE(2) production.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Reperfusion Injury / Brain Ischemia / Intramolecular Oxidoreductases / Cyclooxygenase 2 Type of study: Prognostic_studies Limits: Animals Language: En Journal: Br J Pharmacol Year: 2010 Document type: Article Affiliation country: Japan Country of publication: United kingdom

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Reperfusion Injury / Brain Ischemia / Intramolecular Oxidoreductases / Cyclooxygenase 2 Type of study: Prognostic_studies Limits: Animals Language: En Journal: Br J Pharmacol Year: 2010 Document type: Article Affiliation country: Japan Country of publication: United kingdom